Flexible and expeditious assay for quantitative monitoring of alpha-amylase and amyloglucosidase activities

• Type: Article in International Scientific Journal
• Date: 2019

Authors

Oliveira, HM (Author) Other; Pinheiro, AQ (Author) Other; Antonio J M Fonseca (Author) ICBAS; A. Cabrita (Author) ICBAS; Maia, MRG (Author) ICBAS

Journal

The Journal is awaiting validation by the Administrative Services.

Title: METHODSX

Vol. 6

Pages: 246-258

ISSN: 2215-0161

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 6 Citations

Other information

Authenticus ID: P-00Q-E8D

DOI: 10.1016/j.mex.2019.01.007

Abstract (EN): The monitoring of the activity of alpha-amylase and amyloglucosidase is an important tool for studying their role in the hydrolysis of starch. Here we introduced an improved method capable to measure the activity of alpha-amylase and amyloglucosidase from different sources based on a quantitative starch-iodine assay. The developments of the assay sought the consistent preparation of the reagents, the rescale of the assay and the adjustment of the sensitivity. This was complemented by a glucose yield assay for amyloglucosidase that allowed a secondary source of information when insoluble starches were studied. The proposed method showed high precision in long-term use (RSD <6.3%). Furthermore, all experimental conditions can be adapted according to the equipment available at each laboratory, transforming this method in a broadband analytical tool for screening alpha-amylase and amyloglucosidase activities. Tailorable assay based on the starch-iodine staining for the determination of alpha-amylase and amyloglucosidase activities. Enhanced consistence of reagent preparation. High intra-day and inter-day reproducibility. (C) 2019 The Authors. Published by Elsevier B.V.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13