Miniaturized Fluorimetric Method for Quantification of Zinc in Dry Dog Food

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Martins, RC (Author) Other; Pereira, AM (Author) Other; Matos, E (Author) Other; Barreiros, L (Author) Other; Antonio J M Fonseca (Author) ICBAS; A. Cabrita (Author) ICBAS; Marcela A Segundo (Author) FFUP

Journal

Title: Journal of Analytical Methods in Chemistry

Vol. 2020

Pages: 1-6

ISSN: 2090-8865

Publisher: Hindawi

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 2 Citations

Other information

Authenticus ID: P-00S-S95

DOI: 10.1155/2020/8821809

Abstract (EN): Zinc is an essential trace element for animals in several biological processes, particularly in energy production, and it is acquired from food ingestion. In this context, a microplate-based fluorimetric assay was developed for simple, fast, and low-cost determination of zinc in pet food using 2,2 '-((4-(2,7-difluoro-3,6-dihydroxy-4aH-xanthen-9-yl)-3-methoxyphenyl)azanediyl)diacetic acid (FluoZin-1) as fluorescent probe. Several aspects were studied, namely, the stability of the fluorescent product over time, the FluoZin-1 concentration, and the pH of reaction media. The developed methodology provided a limit of detection of 1 mu g L(-1)in sample acid digests, with a working range of 10 to 200 mu g L-1, corresponding to 100-2000 mg of Zn per kg of dry dog food samples. Intraday repeatability and interday repeatability were assessed, with relative standard deviation values < 3.4% (100 mu g L-1) and <11.7% (10 mu g L-1). Sample analysis indicated that the proposed fluorimetric assay provided results consistent with ICP-MS analysis. These results demonstrated that the developed assay can be used for rapid determination of zinc in dry dog food.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6