Identification of immunoreactive extracellular proteins of Streptococcus agalactiae in bovine mastitis

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Trigo, G (Author) Other; Paula Ferreira (Author) ICBAS; Ribeiro, N (Author) Other; Dinis, M (Author) Other; Andrade, EB (Author) Other; Melo Cristino, J (Author) Other; Ramirez, M (Author) Other; Tavares, D (Author) Other

Journal

Title: Canadian Journal of Microbiology

Vol. 54

Pages: 899-905

ISSN: 0008-4166

Publisher: NRC Research Press

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 6 Citations

Other information

Authenticus ID: P-003-V11

DOI: 10.1139/w08-083

Abstract (EN): Streptococcus agalactiae is a common pathogen that causes bovine mastitis. The aims of this study were to evaluate the antibody response against S. agalactiae extracellular proteins in the whey and serum of naturally infected bovines and to identify possible immunodominant extracellular antigens. IgG(1) antibodies against S. agalactiae extracellular proteins were elevated in the whey and serum of naturally infected bovines. In the whey, the levels of IgG(1) specific for S. agalactiae extracellular proteins were similar in infected and noninfected milk quarters from the same cow, and the production of antibodies specific for S. agalactiae extracellular proteins was induced only by infection with this bacterium. The immunoreactivity of extracellular proteins with bovine whey was clearly different in infected versus control animals. Group B protective surface protein and 5'-nucleotidase family protein were 2 major immunoreactive proteins that were detected only in the whey of infected cows, suggesting that these proteins may be important in the pathogenesis of S. agalactiae-induced mastitis. This information could be used to diagnose S. agalactiae infection. In addition, these antigens may be useful as carrier proteins for serotype-specific polysaccharides in conjugate vaccines.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7