Glutathione is necessary to ensure benefits of calorie restriction during ageing in Saccharomyces cerevisiae

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Mannarino, SC (Author) Other; Amorim, MA (Author) Other; Pereira, MD (Author) Other; Pedro Moradas Ferreira (Author) ICBAS; Panek, AD (Author) Other; Vitor Costa (Author) ICBAS; Eleutherio, ECA (Author) Other

Journal

Title: Mechanisms of Ageing and Development

Vol. 129

Pages: 700-705

ISSN: 0047-6374

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 19 Citations

Scopus - 22 Citations

Other information

Authenticus ID: P-003-TNW

DOI: 10.1016/j.mad.2008.09.001

Abstract (EN): Calorie restriction increases longevity of mammals and yeasts but this mechanism remains unclear. In this Study, the role of glutathione on lifespan extension induced by calorie restriction was investigated by using a Saccharomyces cerevisiae strain deficient in glutathione synthesis (gsh1). We observed all increase in chronological lifespan of calorie-restricted gsh1 mutant cells, compared to WT (wild type) strain, which was associated with a reduction in the levels of oxidative stress biomarkers. The gsh1 strain showed all increase in cell yield under calorie restriction that was associated with a higher pyruvate kinase activity and a reduction in oxygen consumption and aconitase activity. This indicates that the respiratory metabolism is decreased in gsh1 mutant cells. The lifespan extension of gsh1 Mutant cells did not represent an advantage at long term, since old cells of gsh1 strain showed a higher frequency of petite mutants. In addition, aged WT cells outlast aged gsh1 mutant cells in direct competition assays ill a fresh medium. These results Suggest that glutathione is required for the beneficial effects of caloric restriction on cellular longevity.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6