Feeding frequency and dietary protein/carbohydrate ratio affect feed intake and appetite regulation-related genes expression in gilthead seabream (Sparus aurata)

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Basto-Silva, C (Author) Other; Couto, A (Author) Other; Rodrigues, J (Author) Other; Oliva Teles, A (Author) FCUP; Navarro, I (Author) Other; Kaiya, H (Author) Other; Capilla, E (Author) Other; Guerreiro, I (Author) Other

Journal

Title: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Vol. 267

ISSN: 1095-6433

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 0 Citations

Other information

Authenticus ID: P-00W-6J0

DOI: 10.1016/j.cbpa.2022.111168

Abstract (EN): To evaluate the effects of feeding frequency (FF) and dietary protein/carbohydrate (P/CH) ratios on appetite regulation of gilthead seabream, two practical diets were formulated to include high protein and low carbohydrate (P50/CH10 diet) or low protein and high carbohydrate (P40/CH20 diet) content and each diet was fed to triplicate groups of fish until visual satiation each meal at a FF of 1, 2, or 3 meals per day. Feed intake and feed conversion ratio were higher in fish fed 2 or 3 meals than 1 meal per day and in fish fed the P40/CH20 than the P50/CH10 diet. The specific growth rate was only affected by FF, being higher in fish fed 2 or 3 meals per day than 1 meal per day. Expression of the cocaine-amphetamine-related transcript, corticotropin-releasing hormone, ghrelin receptor-a (ghsr-a), leptin, and neuropeptide yin the brain, cholecystokinin (cck) in the intestine, and leptin and ghrelin in the stomach was not affected by FF or dietary P/CH ratio. This is the first time that ghrelin cells were immune-located in the stomach of gilthead seabream. Fish fed 3 meals per day presented lower cck expression in the brain than those fed twice per day and higher hepatic ghsr-b expression than those fed once per day. Fish fed P40/CH20 diet presented higher hepatic leptin expression than those fed P50/CH10 diet. In conclusion, present results indicate that feeding a P40/CH20 diet at 3 meals a day seems to decrease the satiation feeling of gilthead seabream compared to fish fed higher P/CH ratio diets or fed 1 or 2 meals a day.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9