Novel protein carrier system based on cyanobacterial nano-sized extracellular vesicles for application in fish

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Matinha Cardoso, J (Author) Other; Coutinho, F (Author) Other; Lima, S (Author) Other; Eufrasio, A (Author) Other; Carvalho, AP (Author) FCUP; Oliva Teles, A (Author) FCUP; Bessa, J (Author) Other; Paula Tamagnini (Author) FCUP; Claudia Alexandra dos Reis Serra (Author) FCUP; Oliveira, P (Author) FCUP

Journal

Title: Microbial Biotechnology

Vol. 15

Pages: 2191-2207

ISSN: 1751-7907

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 1 Citation

Scopus - 10 Citations

Other information

Authenticus ID: P-00W-ECJ

DOI: 10.1111/1751-7915.14057

Abstract (EN): Aquaculture has been one of the fastest-growing food industry sectors, expanding at the pace of consumers' demands. To promote safe and effective fish growth performance strategies, and to stimulate environmentally friendly solutions to protect fish against disease outbreaks, new approaches are needed to safeguard fish welfare, as well as farmers and consumers interests. Here, we tested the use of cyanobacterial extracellular vesicles (EVs) as a novel nanocarrier system of heterologous proteins for applications in fish. We started by incubating zebrafish larvae with Synechocystis sp. PCC6803 EVs, isolated from selected mutant strains with different cell envelope characteristics. Results show that Synechocystis EVs are biocompatible with fish larvae, regardless of their structural composition, as EVs neither induced fish mortality nor triggered significant inflammatory responses. We establish also that cyanobacteria are amenable to engineering heterologous protein expression and loading into EVs, for which we used the reporter sfGFP. Moreover, upon immersion treatment, we successfully demonstrate that sfGFP-loaded Synechocystis EVs accumulate in the gastrointestinal tract of zebrafish larvae. This work opens the possibility of using cyanobacterial EVs as a novel biotechnological tool in fish, with prospective applications in carrying proteins/enzymes, for example for modulating their nutritional status or stimulating specific adaptive immune responses.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 17