Environmentally relevant dose of the endocrine disruptor tributyltin disturbs redox balance in female thyroid gland

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Andrade, MN (Author) Other; Melo Paiva, FD (Author) Other; Teixeira, MP (Author) Other; de Lima, NC (Author) Other; Soares, P (Author) FMUP; Graceli, JB (Author) Other; de Carvalho, DP (Author) Other; Morris, EAR (Author) Other; Ferreira, ACF (Author) Other; Miranda Alves, L (Author) Other

Journal

Title: Molecular and Cellular Endocrinology

Vol. 553

ISSN: 0303-7207

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 0 Citations

Other information

Authenticus ID: P-00W-STV

DOI: 10.1016/j.mce.2022.111689

Abstract (EN): Tributyltin (TBT) is an endocrine disruptor used as a biocide in nautical paints. Even though many TBT effects in marine species are known, data in mammals are scarce, especially regarding the thyroid gland. The present study aimed to evaluate the effect of a subchronic exposure to TBT on thyroid oxidative stress of female Wistar rats. Rats received vehicle (control group), 200 or 1000 ng TBT/kg body weight/day for 40 days. After euthanasia, one part of the thyroids were collected in order to assess iodide uptake; activity and/or mRNA expression of thyroperoxidase (TPO) and dual oxidases (DUOXs); activity and/or mRNA expression of catalase, glutathione peroxidase, superoxide dismutase and NADPH oxidase 4 (CAT, GPx, SOD and NOX4); 4-hydroxynonenal (4-HNE) expression and total thiol groups levels; and mRNA expression of estrogen receptors alpha and beta (ER alpha and ER beta). The remaining part of the thyroid was processed for morphological analysis of estrogen receptor alpha (ER alpha) and for collagen deposition. Iodide uptake was not changed with treatments. TPO activity and expression were increased in the TBT1000 group (259.81% and 95.17%). The activity, but not mRNA, of CAT (17.36% TBT200; 27.10% TBT1000) and GPx (29.24% TBT200; 28.97% TBT1000) were decreased by TBT. SOD and NADPH oxidase activity, as well as thiol group and 4-HNE levels remained unchanged. Interstitial collagen deposition increased in the TBT200 group (39.54%). The mRNA expression of ER alpha increased in TBT-treated rats (44.87% TBT200; 36.43% TBT1000), while protein expression was increased but not reaching significance (TBT1000, p = 0.056) by TBT. Therefore, our results show that TBT increases TPO expression and reduces antioxidant enzyme activities in the thyroid gland leading to oxidative stress. Some of these effects could be mediated by the ER alpha pathway.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10