Exosomal glypican-1 discriminates pancreatic ductal adenocarcinoma from chronic pancreatitis

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Moutinho Ribeiro, P (Author) Other; Adem, B (Author) Other; Batista, I (Author) Other; Silva, M (Author) Other; Silva, S (Author) Other; Ruivo, CF (Author) Other; Morais, R (Author) Other; Peixoto, A (Author) Other; Coelho, R (Author) Other; Costa Moreira, P (Author) Other; Lopes, S (Author) Other; Vilas Boas, F (Author) Other; Duraes, C (Author) Other; Lopes, J (Author) Other; Barroca, H (Author) Other; Carneiro F (Author) FMUP; Melo, SA (Author) Other; Macedo G (Author) FMUP

Journal

Title: Digestive and Liver Disease

Vol. 54

Pages: 871-877

ISSN: 1590-8658

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 12 Citations

Other information

Authenticus ID: P-00V-VPP

DOI: 10.1016/j.dld.2021.10.012

Abstract (EN): Background and aims: Pancreatic ductal adenocarcinoma (PDAC) diagnosis can be difficult in a chronic pancreatitis (CP) background, especially in its mass forming presentation. We aimed to assess the accuracy of glypican-1-positive circulating exosomes (GPC1+ crExos) to distinguish PDAC from CP versus the state-of-the-art CA 19-9 biomarker. Methods: This was a unicentric prospective cohort. Endoscopic ultrasound with fine-needle aspiration or biopsy and blood tests (GPC1+ crExos and serum CA 19-9) were performed. Results: The cohort comprised 60 PDAC and 29 CP (7 of which mass forming - MF) patients. Median levels of GPC1+ crExos were significantly higher in PDAC (99.7%) versus CP (28.4%; p<0.00 01) with an AUROC of 0.96 with 98.3% sensitivity and 86.2% specificity for a cut-offof 45.0% (p<0.0 001); this outperforms CA 19-9 AUROC of 0.82 with 78.3% sensitivity and 65.5% specificity at a cut-offof 37 U/mL (p<0.0 001). The superiority of% GPC1+crExos over CA 19-99 in differentiating PDAC from CP was observed in both early (stage I) and advanced tumors (stages II-IV). Conclusion: Levels of GPC1+ crExos coupled to beads enable differential diagnosis between PDAC and CP including its mass-forming presentation.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7