Microfluidic-based platform to mimic the in vivo peripheral administration of neurotropic nanoparticles

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Lopes, CDF (Author) Other; Gomes, CP (Author) Other; Neto, E (Author) Other; Sampaio, P (Author) Other; Paulo Aguiar (Author) FMUP; Pego, AP (Author) Other

Journal

Title: Nanomedicine

Vol. 11

Pages: 3205-3221

ISSN: 1743-5889

Publisher: Future Medicine Ltd.

Indexing

ISI Web of Knowledge - 9 Citations

Scopus - 8 Citations

Other information

Authenticus ID: P-00M-75Q

DOI: 10.2217/nnm-2016-0247

Abstract (EN): Aim: Propose a nanoparticle for neuron-targeted retrograde gene delivery and describe a microfluidic-based culture system to provide insight into vector performance and safety. Methods: Using compartmentalized neuron cultures we dissected nanoparticle bioactivity upon delivery taking advantage of (quantitative) bioimaging tools. Results: Targeted and nontargeted nanoparticles were internalized at axon terminals and retrogradely transported to cell bodies at similar average velocities but the former have shown an axonal flux 2.7-times superior to nontargeted nanoparticles, suggesting an improved cargo-transportation efficiency. The peripheral administration of nanoparticles to axon terminals is nontoxic as compared with their direct administration to the cell body or whole neuron. Conclusion: A neuron-targeted nanoparticle system was put forward. Microfluidic-based neuron cultures are proposed as a powerful tool to investigate nanoparticle bio-performance.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 17