DNA Mimics for the Rapid Identification of Microorganisms by Fluorescence in situ Hybridization (FISH)

• Type: Another Publication in an International Scientific Journal
• Date: 2008

Authors

Cerqueira, L. (Author) Other; Nuno F Azevedo (Author) Other; Carina Almeida (Author) Other; Tatiana Jardim (Author) Other; Charles William Keevil (Author) Other; Maria J Vieira (Author) Other

Journal

Title: International Journal Of Molecular Sciences

Vol. 9

Pages: 1944-1960

ISSN: 1661-6596

Publisher: MDPI

Indexing

ISI Web of Knowledge - 63 Citations

ISI Web of Science

Scopus - 76 Citations

INSPEC

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-003-VVD

DOI: 10.3390/ijms9101944

Abstract (EN): Fluorescence in situ hybridization (FISH) is a well-established technique that is used for a variety of purposes, ranging from pathogen detection in clinical diagnostics to the determination of chromosomal stability in stem cell research. The key step of FISH involves the detection of a nucleic acid region and as such, DNA molecules have typically been used to probe for the sequences of interest. However, since the turn of the century, an increasing number of laboratories have started to move on to the more robust DNA mimics methods, most notably peptide and locked nucleic acids (PNA and LNA). In this review, we will cover the state-of-the-art of the different DNA mimics in regard to their application as efficient markers for the presence of individual microbial cells, and consider their potential advantages and pitfalls. Available PNA probes are then reassessed in terms of sensitivity and specificity using rRNA databases. In addition, we also attempt to predict the applicability of DNA mimics in well-known techniques attempting to detect in situ low number of copies of specific nucleic acid sequences such as catalyzed reporter deposition (CARD) and recognition of individual genes (RING) FISH.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 17