Microsequential injection analysis/lab-on-valve system for the automatic evaluation of acetylcholinesterase inhibitors

• Type: Article in International Scientific Journal
• Date: 2021

Authors

Ndongo, TM (Author) Other; Passos, MLC (Author) Other; Duraes, F (Author) Other; Resende, DISP (Author) Other; Pinto, M (Author) Other; Emilia Sousa (Author) FFUP; Lucia L M F S Saraiva (Author) FFUP

Journal

Title: Archiv der Pharmazie

Vol. 354

ISSN: 0365-6233

Publisher: Wiley-Blackwell

Indexing

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Other information

Authenticus ID: P-00V-1E6

DOI: 10.1002/ardp.202100150

Abstract (EN): A miniaturized microsequential injection/lab-on-valve (mu SIA-LOV) system was developed and shown to be a useful alternative to perform inhibitory studies on acetylcholinesterase. These studies are essential for the evaluation of the potential therapeutic effect of drugs commonly used in the treatment of Alzheimer's disease. Donepezil, galantamine, and rivastigmine were tested, in addition to compounds based on the xanthone scaffold. Four of these xanthone derivatives were identified as having EC50 values between 676 and 4466 mu mol/l, showing a potential inhibitory effect higher than the clinical agent rivastigmine. The developed automatic system added advantages of reduction of reagents and sample consumption (around 55 mu l per analysis), lower cost per analysis, and the generation of less waste (around 1.2 ml per analysis). The mu SIA-LOV system is also a robust, rapid, reliable, and simple system to use. Docking studies suggested a possible mode of interaction with the target acetylcholinesterase protein.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11