Nanocrystalline diamond: In vitro biocompatibility assessment by MG63 and human bone marrow cells cultures

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Amaral, M (Author) Other; Dias, AG (Author) Other; Gomes, PS (Author) FMDUP; Lopes, MA (Author) FEUP; Silva, RF (Author) Other; Santos, JD (Author) FEUP; Fernandes, MH (Author) FMDUP

Journal

Title: Journal of Biomedical Materials Research - Part A

Vol. 87A

Pages: 91-99

ISSN: 1549-3296

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 83 Citations

ISI Web of Science

Scopus - 109 Citations

COMPENDEX

Scientific classification

FOS: Engineering and technology > Environmental biotechnology

CORDIS: Health sciences

Other information

Authenticus ID: P-003-VVR

DOI: 10.1002/jbm.a.31742

Abstract (EN): Nanocrystalline diamond (NCD) has a great potential for prosthetic implants coating. Nevertheless, its biocompatibility still has to be better understood. To do so, we employed several materials characterization techniques (SEM, AFM, micro-Raman spectroscopy) and cell Culture assays using MG63 osteoblast-like and human bone marrow, cells. Biochemical routines (MTT assays, Lowry's method, ALP activity) supported by SEM and confocal microscopy characterization were carried out. We used silicon nitride (Si3N4) Substrates for NCD coatings based on a previous demonstration Of the Superior adhesion and tribological performance of these NCD coated ceramics. Results demonstrate an improved human osteoblast proliferation and the stimulation of differentiated markers, like ALP activity and matrix mineralization, compared with standard polystyrene tissue Culture plates. The nanometric featuring of NCD, associated to its chemical affinity are key points for bone regeneration purposes. (C) 2007 Wiley Periodicals, Inc.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9