Correlations between distribution coefficients of various biomolecules in different polymer/polymer aqueous two-phase systems

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Pedro P. Madeira (Author) FEUP; José A. Teixeira (Author) FEUP; Eugénia A. Macedo (Author) FEUP; Larissa M. Mikheeva (Author) FEUP; Boris Y. Zaslavsky (Author) FEUP

Journal

Title: Fluid Phase Equilibria

Vol. 267

Pages: 150-157

ISSN: 0378-3812

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 37 Citations

ISI Web of Science

Scopus - 38 Citations

Scientific classification

FOS: Engineering and technology > Chemical engineering

CORDIS: Technological sciences > Engineering > Chemical engineering ; Technological sciences > Technology

Other information

Authenticus ID: P-003-ZBQ

DOI: 10.1016/j.fluid.2008.03.003

Abstract (EN): Distribution coefficients for a variety of proteins and certain other biomolecules (peptides, amino acids, and carbohydrates) (overall 27 different solutes) were measured in aqueous two-phase systems (ATPSs) dextran (Dex)-polyethylene glycol (PEG) and Dex-Ucon 50-HB-5100 (Ucon-a random copolymer of ethylene glycol and propylene glycol) both containing 0.15 M NaCl in 0.01 M phosphate buffer, pH 7.4, at 23 degrees C. Distribution coefficients of some selected solutes were also measured in the above two-phase systems at three different polymer concentrations for each system. It was established that the distribution coefficients for all the proteins examined in the ATPSs are correlated according to the so-called Collander linear equation.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8