Crosstalk between GH/IGF-I axis and steroid hormones (progesterone, 17 beta-estradiol) in canine mammary tumours

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Queiroga, FL (Author) Other; Perez Alenza, MD (Author) Other; Silvan, G (Author) Other; Pena, L (Author) Other; Lopes, C (Author) ICBAS; Illera, JC (Author) Other

Journal

Title: Journal of Steroid Biochemistry and Molecular Biology

Vol. 110

Pages: 76-82

ISSN: 0960-0760

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 28 Citations

Scopus - 38 Citations

Other information

Authenticus ID: P-003-ZTF

DOI: 10.1016/j.jsbmb.2008.02.005

Abstract (EN): Growth hormone (GH), insulin-like growth factor I (IGF-I), progesterone (P4) and 17 beta-estradiol (17-E2) concentrations have been studied in 84 mammary tumours (44 dysplasias and benign tumours and 40 malignant neoplasias) from 33 female dogs. Thirteen normal mammary glands from 80 healthy female dogs were also analysed as controls. GH concentrations were determined in mammary homogenates by radio-immunoassay. IGF-I, P4 and 17-E2 tissue levels were determined by enzyme-immunoassay (EIA) techniques. The potential correlations between GH/IGF-I concentrations and P4 and 17-E2 mammary tissue levels were investigated. Tissue GH (p < 0.01) and IGF-I concentrations (p < 0.01) were significantly higher in malignant tumours than in benign neoplasms. Likewise, malignant tumours were the mammary lesions that displayed the highest P4 and 17-E2 tissue levels. Strong correlations between GH/IGF-I (n = 84; r= 0.436; p < 0.001), P4/GH (n = 84; r= 0.562; p < 0.001) and 17-E2/IGF-I (n = 84; r= 0.638; p < 0.001) were observed in tumoral tissue homogenates. Our study provides evidence that P4 might increase autocrine GH production which might directly stimulate local or systemic IGF-I secretion. Additionally, the IGF-I effect might be influenced by local levels of 17-E2. These results suggest that all these hormones and factors might act as local growth factors stimulating the development and/or maintenance of canine mammary tumours in an autocrine/paracrine manner.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7