Multiplexing Y chromosome STRs: Analysis of artifactual bands and PCR strategies

• Type: Article in International Conference Proceedings Book
• Date: 2000

Authors

Gonzalez Neira, A (Author) Other; Gusmao, L (Author) FCUP; Barral, S (Author) Other; Lareu, MV (Author) Other; Carracedo, A (Author) Other

Conference proceedings International

Title: PROGRESS IN FORENSIC GENETICS 8

Pages: 436-438

18th Congress of the International-Society-for-Forensic-Haemogenetics (ISFH)

SAN FRANCISCO, CA, AUG 17-21, 1999

Indexing

ISI Web of Knowledge - 0 Citations

Other information

Authenticus ID: P-008-KNF

Abstract (EN): Different technical conditions were tried to optimized a multiplex system to amplify five Y-specific STR loci. DYS19, DYS389 I, DYS389 II, DYS390 and DYS393. The pentaplex was evaluated for typing using two different platforms, ABI Prism 377 and Automatic Laser Fluorescent (ALF) DNA sequencer. The designed pentaplex has proved to be very useful for population genetic studies and forensic applications allowing the analysis using a reduced amount of sample. Moreover, a combination is possible in the same lane of other Y STR singleplex or multiplexes, especially when typed using different fluorescent dyes.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 3