Hydrodynamic Effects on Biofilm Development and Recombinant Protein Expression

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Soares, A (Author) Other; Luciana Gomes (Author) FEUP; Monteiro, GA (Author) Other; Filipe Mergulhão (Author) FEUP

Journal

Title: Microorganisms

Vol. 10

Final page: 931

Publisher: MDPI

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 0 Citations

Associated Projects

ALiCE - ALiCE - Laboratório Associado em Engenharia Química

LEPABE - Financiamento Plurianual 2020-2023 da Unidade de I&D LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

FilmProt - Utilização de biofilmes de Escherichia coli para a produção de proteínas recombinantes e outros compostos de elevado valor acrescentado

Other information

Authenticus ID: P-00W-FPV

DOI: 10.3390/microorganisms10050931

Abstract (EN): Hydrodynamics play an important role in the rate of cell attachment and nutrient and oxygen transfer, which can affect biofilm development and the level of recombinant protein production. In the present study, the effects of different flow conditions on the development of Escherichia coli biofilms and the expression of a model recombinant protein (enhanced green fluorescent protein, eGFP) were examined. Planktonic and biofilm cells were grown at two different flow rates in a recirculating flow cell system for 7 days: 255 and 128 L h(-1) (corresponding to a Reynolds number of 4600 and 2300, respectively). The fluorometric analysis showed that the specific eGFP production was higher in biofilms than in planktonic cells under both hydrodynamic conditions (3-fold higher for 255 L h(-1) and 2-fold higher for 128 L h(-1)). In the biofilm cells, the percentage of eGFP-expressing cells was on average 52% higher at a flow rate of 255 L h(-1). Furthermore, a higher plasmid copy number (PCN) was obtained for the highest flow rate for both planktonic (244 PCN/cell versus 118 PCN/cell) and biofilm cells (43 PCN/cell versus 29 PCN/cell). The results suggested that higher flow velocities promoted eGFP expression in E. coli biofilms.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10