"On the Collander equation": Protein partitioning in polymer/polymer aqueous two-phase systems

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Pedro P. Madeira (Author) FEUP; José A. Teixeira (Author) FEUP; Eugénia A. Macedo (Author) FEUP; Larissa M. Mikheeva (Author) FEUP; Boris Y. Zaslavsky (Author) FEUP

Journal

Title: Journal of Chromatography A

Vol. 1190

Pages: 39-43

ISSN: 0021-9673

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 33 Citations

ISI Web of Science

Scopus - 34 Citations

COMPENDEX

Scientific classification

FOS: Engineering and technology > Chemical engineering

Other information

Authenticus ID: P-003-ZEA

DOI: 10.1016/j.chroma.2008.03.003

Abstract (EN): Distribution coefficients of randomly selected proteins were measured in aqueous two-phase systems (ATPSs) formed by different combinations of Dextran-75 (Dex), Ficoll-70, polyethylene glycol-8000 (PEG), hydroxypropyl starch-100 (PES), and Ucon50HB5100 (Ucon, a random copolymer of ethylene glycol and propylene glycol) at particular polymer concentrations, all containing 0.15 M NaCl in 0.01 M phosphate buffer, pH 7.4. Most of the proteins in the PEG-Ucon system precipitated at the interface. In the other ATPSs, namely, PES-PEG, PES-Ucon, Ficoll-PEG, Ficoll-Ucon, and in Dex-PEG and Dex-Ucon described earlier the distribution coefficients for the proteins were correlated according to the solvent regression equation: In K-i = a(io) In K-o + b(io) where K-i and K-o are the distribution coefficients for any protein in the ith and oth two-phase systems. Coefficients a(io) and b(io) are constants, the values of which depend upon the particular compositions of the two-phase systems under comparison. (C) 2008 Published by Elsevier B.V.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5