The pro-proliferative effect of insulin in human breast epithelial DMBA-transformed and non-transformed cell lines is PI3K-, mTOR- and GLUT1-dependent

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Silva, C (Author) FMUP; Andrade, N (Author) Other; guimaraes, jt (Author) FMUP; Cardoso, E (Author) FMUP; Meireles, C (Author) Other; Pinto, V (Author) Other; Paiva, J-A (Author) FMUP; Martel, F (Author) FMUP

Journal

Title: Cell Biochemistry and Function

Vol. 40

Pages: 127-137

ISSN: 0263-6484

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 0 Citations

Other information

Authenticus ID: P-00V-YK6

DOI: 10.1002/cbf.3681

Abstract (EN): Type 2 diabetes mellitus (T2DM) is linked to an increased risk of breast cancer. We aimed to investigate how T2DM-associated characteristics (high levels of glucose, insulin, leptin, inflammatory mediators and oxidative stress) influence breast cancer carcinogenesis, in DMBA-treated (MCF-12A(DMBA)) and non-treated breast epithelial (MCF-12A) cell lines. Insulin (50 nM) promotes cell proliferation, H-3-DG uptake and lactic acid production in both cell lines. The stimulatory effects of insulin upon cell proliferation and H-3-DG uptake were hampered by rapamycin, LY294001 and BAY-876, in both cell lines. In conclusion, hyperinsulinemia, one important characteristic of T2DM, contributes to the initiation of breast cancer by a PI3K- and mTOR-dependent mechanism involving increased GLUT1-mediated glucose uptake. Significance The pro-proliferative effect of insulin in human breast epithelial DMBA-transformed and non-transformed cell lines is PI3K-, mTOR- and GLUT1-dependent.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11