Automated Identification and Localization of Hematopoietic Stem Cells in 3D Intravital Microscopy Data

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Khorshed, RA (Author) Other; Hawkins, ED (Author) Other; Duarte, D (Author) FMUP; Scott, MK (Author) Other; Akinduro, OA (Author) Other; Rashidi, NM (Author) Other; Spitaler, M (Author) Other; Lo Celso, C (Author) Other

Journal

Title: Stem Cell Reports

Vol. 5

Pages: 139-153

ISSN: 2213-6711

Publisher: Elsevier

Indexing

Scopus - 19 Citations

Other information

Authenticus ID: P-00T-90Q

DOI: 10.1016/j.stemcr.2015.05.017

Abstract (EN): Measuring three-dimensional (3D) localization of hematopoietic stem cells (HSCs) within the bone marrow microenvironment using intravital microscopy is a rapidly expanding research theme. This approach holds the key to understanding the detail of HSC-niche interactions, which are critical for appropriate stem cell function. Due to the complex tissue architecture of the bone marrow and to the progressive introduction of scattering and signal loss at increasing imaging depths, there is no ready-made software to handle efficient segmentation and unbiased analysis of the data. To address this, we developed an automated image analysis tool that simplifies and standardizes the biological interpretation of 3D HSC microenvironment images. The algorithm identifies HSCs and measures their localization relative to surrounding osteoblast cells and bone collagen. We demonstrate here the effectiveness, consistency, and accuracy of the proposed approach compared to current manual analysis and its wider applicability to analyze other 3D bone marrow components. © 2015 The Authors.

Language: English

Type (Professor's evaluation): Scientific