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A quantitative ELISA for monitoring the secretion of ZZ-fusion proteins using SpA domain as immunodetection reporter system

Title
A quantitative ELISA for monitoring the secretion of ZZ-fusion proteins using SpA domain as immunodetection reporter system
Type
Article in International Scientific Journal
Year
2001
Authors
Mergulhao, FJM
(Author)
Other
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Monteiro, GA
(Author)
Other
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Cabral, JMS
(Author)
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Taipa, MA
(Author)
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Journal
Vol. 19
Pages: 239-244
ISSN: 1073-6085
Publisher: Springer Nature
Indexing
Scientific classification
FOS: Natural sciences > Biological sciences
CORDIS: Technological sciences > Technology > Biotecnology
Other information
Authenticus ID: P-000-SYK
Abstract (EN): A sandwich-type enzyme-linked immunosorbent assay (ELISA) was established for monitoring the secretion of ZZ-fusion proteins. Two antibodies, a monoclonal mouse anti-human proinsulin and a rabbit antibovine IgG (strongly binding to the ZZ-domain), were used to quantify the secretion of recombinant human ZZ-proinsulin to the growth medium of Escherichia coli cultures. The method here reported conjugates the advantages of sandwich-type ELISA assays, namely, high sensitivity, specificity, and throughput, with the possibility of quantifying small protein molecules (e.g., peptides). A further advantage of gene fusion techniques integrating both downstream processing and product detection and quantitation is highlighted. The method is capable of detecting levels of 0.05 ng of ZZ-proinsulin.
Language: English
Type (Professor's evaluation): Scientific
No. of pages: 6
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