Analysis of factors affecting the periplasmic production of recombinant proteins in Escherichia coli

• Type: Article in International Scientific Journal
• Date: 2007

Authors

Filipe J Mergulhao (Author) FEUP; Gabriel A Monteiro (Author) Other

Journal

Title: Journal of Microbiology and Biotechnology

Vol. 17 No. 8

Pages: 1236-1241

ISSN: 1017-7825

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 16 Citations

ISI Web of Science

Scopus - 23 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

CORDIS: Technological sciences > Technology > Biotecnology

Other information

Authenticus ID: P-004-8HS

Abstract (EN): Five fusion proteins between Z domains derived from Staphylococcal Protein A and Green Fluorescent Protein or Human Proinsulin were produced on the periplasm of Escherichia coli. The effects of the molecular weight and amino acid composition of the translocated peptide, culture medium composition, and growth phase of the bacterial culture were analyzed regarding the expression and periplasmic secretion of the recombinant proteins. It was found that secretion was not affected by the size of the translocated peptide (1742 kDa) and that the highest periplasmic production values were obtained on the exponential phase of growth. Moreover, the highest periplasmic values were obtained in minimal medium, showing the relevance of the culture medium composition on secretion. In silico prediction analysis suggested that with respect to the five proteins used in this study, those that are prone to form a-helix structures are more translocated to the periplasm.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6