Microtubule detyrosination guides chromosomes during mitosis

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Barisic, M (Author) Other; Silva e Sousa, RSE (Author) Other; Tripathy, SK (Author) Other; Magiera, MM (Author) Other; Zaytsev, AV (Author) Other; Pereira, AL (Author) Other; Janke, C (Author) Other; Grishchuk, EL (Author) Other; Helder Maiato (Author) FMUP

Journal

Title: Science

Vol. 348

Pages: 799-803

ISSN: 0036-8075

Publisher: American Association for the Advancement of Science

Indexing

ISI Web of Knowledge - 136 Citations

Scopus - 141 Citations

Other information

Authenticus ID: P-00G-47D

DOI: 10.1126/science.aaa5175

Abstract (EN): Before chromosomes segregate into daughter cells, they align at the mitotic spindle equator, a process known as chromosome congression. Centromere-associated protein E (CENP-E)/Kinesin-7 is a microtubule plus-end-directed kinetochore motor required for congression of pole-proximal chromosomes. Because the plus-ends of many astral microtubules in the spindle point to the cell cortex, it remains unknown how CENP-E guides pole-proximal chromosomes specifically toward the equator. We found that congression of pole-proximal chromosomes depended on specific posttranslational detyrosination of spindle microtubules that point to the equator. In vitro reconstitution experiments demonstrated that CENP-E-dependent transport was strongly enhanced on detyrosinated microtubules. Blocking tubulin tyrosination in cells caused ubiquitous detyrosination of spindle microtubules, and CENP-E transported chromosomes away from spindle poles in random directions. Thus, CENP-E-driven chromosome congression is guided by microtubule detyrosination.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5