Improved kymography tools and its applications to mitosis

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Pereira, AJ (Author) Other; Helder Maiato (Author) FMUP

Journal

Title: Methods

Vol. 51

Pages: 214-219

ISSN: 1046-2023

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 23 Citations

Scopus - 24 Citations

Other information

Authenticus ID: P-003-6EG

DOI: 10.1016/j.ymeth.2010.01.016

Abstract (EN): Although applicability of kymographs is limited to nearly one-dimensional (1D) processes, they have been instrumental in the analysis and interpretation of a wide range of dynamic biological processes. We focus here on some applications of kymography in the study of one among the range of 'nearly-1D' processes mitosis. Using this biological context, we suggest generalized procedures in kymograph assembly that allow a partial retrieval of spatial information which is typically lost or distorted in conventional kymography. These kymograph variations, namely guided-kymography and chromo-kymography, are helpful in the determination of actual velocities and discrimination of structures when using thick regions of interest (ROIs). The method used to generate chromo-kymographs is generalized to other (non-kymograph) projection techniques, which include time-stack and z-stack projections.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6