Three-dimensional distribution of GFP-labeled Pseudomonas putida during biofilm formation on solid PAHs assessed by confocal laser scanning microscopy

• Type: Article in International Scientific Journal
• Date: 2003

Authors

A. C. Rodrigues (Author) Other; S. Wuertz (Author) Other; A. G. Brito (Author) Other; Luís Ferreira Melo (Author) FEUP

Journal

Title: Water Science and Technology

Vol. 47 No. 5

Pages: 139-142

ISSN: 0273-1223

Publisher: IWA PUBLISHING

Indexing

ISI Web of Knowledge - 6 Citations

ISI Web of Science

Scopus - 9 Citations

COMPENDEX

Other information

Authenticus ID: P-000-KRM

DOI: 10.2166/wst.2003.0302

Abstract (EN): Confocal laser scanning microscopy was used to monitor the colonization pattern of the gfp-labeled derivative strain of Pseudomonas putida ATCC 17514 on fluorene and phenanthrene crystals. The in situ experiments showed that P. putida tends to grow directly on phenanthrene, forming a biofilm on accessible crystalline surfaces. On the other hand, no significant biofilm formation was observed in the presence of fluorene. The results obtained showed that substrate properties affected bacterial strategy regarding uptake.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4