Highly sensitive detection of the MGB1 transcript (mammaglobin) in the peripheral blood of breast cancer patients

• Type: Article in International Scientific Journal
• Date: 2004

Authors

cerveira, n (Author) Other; Torres, L (Author) Other; Rocha, P (Author) Other; Bizarro, S (Author) Other; Pereira, D (Author) Other; Abreu, J (Author) Other; henrique, rmf (Author) ICBAS; Manuel R Teixeira (Author) ICBAS; CASTEDO, S (Author) FMUP

Journal

Title: International Journal of Cancer

Vol. 108

Pages: 592-595

ISSN: 0020-7136

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 29 Citations

Scopus - 32 Citations

Other information

Authenticus ID: P-000-BMQ

DOI: 10.1002/ijc.11609

Abstract (EN): We describe a new one-step RT-PCR assay for the detection of the mammaglobin (MGBI) gene transcript in the peripheral blood of breast cancer patients. With this approach, the MGBI transcript could be detected in the peripheral blood of 22 of 54 (41%) breast cancer patients prior to any therapy. This method, using specific primers for cDNA Synthesis, proved to be more sensitive (10(-6) to 10(-11) ,usually 10(-7)) than previously reported methodologies. This increased sensitivity was achieved without compromising specificity, as the MGBI transcript was not detected in 38 blood samples of healthy donors and in only 1 of 18 blood samples of patients presenting with hematologic malignancies. A positive correlation was seen between MGBI positivity and breast cancer stage: 0/3 (0%) in stage 0, 3/13 (23%) in stage I, 6/17 (35%) in stage II, 5/10 (50%) in stage III, 8/11 (73%) in stage IV (p = 0.003). The prognostic and therapeutic implications of MGBI positivity by one-step RT-PCR in the peripheral blood of breast cancer patients, especially in clinically localized disease (stages I and II), should be evaluated after long-term clinical follow-up of these patients. (C) 2003 Wiley-Liss, Inc.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4