Dopaminergic Receptors and Tyrosine Hydroxylase Expression in Peripheral Blood Mononuclear Cells: A Distinct Pattern in Central Obesity

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Leite, F (Author) Other; Lima, M (Author) Other; Marino, F (Author) Other; Cosentino, M (Author) Other; ribeiro, l (Author) FMUP

Journal

Title: PLoS ONE

Serial No. e0145669 Vol. 11

ISSN: 1932-6203

Publisher: Public Library of Science

Indexing

ISI Web of Knowledge - 13 Citations

Other information

Authenticus ID: P-00K-7WF

DOI: 10.1371/journal.pone.0147483

Abstract (EN): Background Dopamine (DA) may be involved in central obesity (CO), an inflammatory condition, through its role in the central nervous system and in periphery, where it may affect immune cell function through five different DA receptors (DR). Whether dopaminergic pathways in peripheral immune cells are implicated in the inflammatory condition linked to CO is however unknown. Methods In a cohort of blood donors with and without CO, categorized by waist circumference (WC) (CO: WC >= 0.80 m in women and >= 0.94 m in men), we studied the expression of DR and tyrosine hydroxylase (TH), the rate-limiting enzyme in the synthesis of DA, in peripheral blood mononuclear cells (PBMCs) and their relation with anthropometric and metabolic/endocrine and inflammatory parameters. DR D1-5 and TH expression was assessed by semi quantitative real-time PCR. As inflammatory markers we investigated the immunophenotype of monocyte subsets by flow cytometry, staining for CD14, CD16, CD11b and CD36. Results qCO individuals showed higher plasma levels of leptin and higher inflammatory pattern of monocytes compared with non-CO. PBMC expression of DR D2, DRD4 and DR D5 as well as of TH were lower in CO in comparison with non-CO. DR D2, and DR D5 expression correlated with lower WC and weight, and with lower inflammatory pattern of monocytes, and TH expression correlated with lower WC. DR D-4 expression correlated with lower plasma levels of glycosylated hemoglobin, and DR D-2 expression correlated with lower CO. Conclusions Results show that CO is associated with peripheral inflammation and downregulation of dopaminergic pathways in PBMCs, possibly suggesting DR expressed on immune cells as pharmacological targets in obesity for better metabolic outcome.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12