Evaluation of Giardia duodenalis viability after metronidazole treatment by flow cytometry

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Barbosa, J (Author) Other; rodrigues, ag (Author) FMUP; Perez, MJ (Author) Other; pina-vaz, c (Author) FMUP

Journal

Title: Memorias do Instituto Oswaldo Cruz

Vol. 109

Pages: 1078-1080

ISSN: 0074-0276

Publisher: Fundação Oswaldo Cruz (Fiocruz)

Indexing

ISI Web of Knowledge - 1 Citation

Scopus - 2 Citations

Other information

Authenticus ID: P-00A-1B9

DOI: 10.1590/0074-0276140267

Abstract (EN): Giardia duodenalis (syn. lamblia; syn. intestinalis) susceptibility testing is not routinely performed because the classical culture methods are very time-consuming and laborious. We developed a novel flow cytometry (FC) assay to evaluate the susceptibility of G. duodenalis trophozoites to metronidazole (MTZ). Different concentrations of MTZ were added to cultures of trophozoites (105/mL) and the cultures were incubated for different periods. The 50% inhibitory concentration was calculated and propidium iodide (PI) was used to quantify the number of dead cells. After treatment, PI-positive trophozoites increased with increasing drug concentration and exposure time. An excellent correlation was found between FC and the classical method. A novel, accurate and reliable method is now available to evaluate G. duodenalis viability.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 3