L-Threonine Supplementation During Colitis Onset Delays Disease Recovery

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Gaifem, J (Author) Other; Goncalves, LG (Author) Other; Ricardo Jorge Dinis Oliveira (Author) FMUP; Cunha, C (Author) Other; Carvaiho, A (Author) Other; Torrado, E (Author) Other; Rodrigues, F (Author) Other; Saraivans, M (Author) Other; Castrou, AG (Author) Other; Silvestre, R (Author) Other

Journal

Title: Frontiers in Physiology

Vol. 9

ISSN: 1664-042X

Publisher: Frontiers Media

Indexing

ISI Web of Knowledge - 9 Citations

Scopus - 9 Citations

Other information

Authenticus ID: P-00P-KGX

DOI: 10.3389/fphys.2018.01247

Abstract (EN): Dietary nutrients have emerged as potential therapeutic adjuncts for inflammatory bowel disease (IBD) given their impact on intestinal homeostasis through the modulation of immune response, gut microbiota composition and epithelial barrier stability. Several nutrients have already been associated with a protective phenotype. Yet, there is a lack of knowledge toward the most promising ones as well as the most adequate phase of action. To unveil the most prominent therapy candidates we characterized the colon metabolic profile during colitis development. We have observed a twofold decrease in threonine levels in mice subjected to DSS-induced colitis. We then assessed the effect of threonine supplementation in the beginning of the inflammatory process (DSS + Thr) or when inflammation is already established (DSS + Thr D8). Colitis progression was similar between the treated groups and control colitic mice, yet threonine had a surprisingly detrimental effect when administered in the beginning of the disease, with mice displaying a delayed recovery when compared to control mice and mice supplemented with threonine after day 8. Although no major changes were found in their metabolic profile, DSS + Thr mice displayed altered expression in mucin-encoding genes, as well as in goblet cell counts, unveiling an impaired ability to produce mucus. Moreover, IL-22 secretion was decreased in DSS + Thr mice when compared to DSS + Thr D8 mice. Overall, these results suggest that supplementation with threonine during colitis induction impact goblet cell number and delays the recovery period. This reinforces the importance of a deeper understanding regarding threonine supplementation in IBD.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7