Novel whey-derived peptides with inhibitory effect against angiotensin-converting enzyme: In vitro effect and stability to gastrointestinal enzymes

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Tavares, T (Author) Other; Contreras, MD (Author) Other; Amorim, M (Author) Other; Pintado, M (Author) Other; Recio, I (Author) Other; F. Xavier Malcata (Author) Other

Journal

Title: Peptides

Vol. 32 No. 1469-1476

Pages: 1013-1019

ISSN: 0196-9781

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 75 Citations

Scopus - 104 Citations

Other information

Authenticus ID: P-002-RR6

DOI: 10.1016/j.peptides.2011.02.005

Abstract (EN): Whey protein concentrate (WPC) was subjected to enzymatic hydrolysis by proteases from the flowers of Cynara cardunculus, and the resulting angiotensin-converting enzyme (ACE)-inhibitory effect was monitored. The whole WPC hydrolysate exhibited an IC(50) value of 52.9 +/- 2.9 mu g/mL, whereas the associated peptide fraction with molecular weight below 3 kDa scored 23.6 +/- 1.1 mu g/mL The latter fraction was submitted to RP-HPLC, and 6 fractions were resolved that exhibited ACE-inhibitory effects. Among the various peptides found, a total of 14 were identified via sequencing with an ion-trap mass spectrometer. Eleven of these peptides were synthesized de novo - to validate their ACE-inhibitory effect, and also to ascertain their stability when exposed to simulated gastrointestinal digestion. Among them, three novel, highly potent peptides were found, corresponding to alpha-lactalbumin f(16-26) - with the sequence KGYGGVSLPEW, alpha-lactalbumin f(97-104) with DKVGINYW, and beta-lactoglobulin f(33-42) with DAQSAPLRVY; their IC(50) values were as low as 0.80 +/- 0.1, 25.2 +/- 1.0 and 13.0 +/- 1.0 mu g/mL, respectively. None of them remained stable in the presence of gastrointestinal enzymes: they were partially, or even totally hydrolyzed to smaller peptides - yet the observed ACE-inhibitory effects were not severely affected for two of those peptides.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7