Molecular cloning and characterization of sea bass (Dicentrarchus labrax L.) CD8 alpha

• Type: Article in International Scientific Journal
• Date: 2006

Authors

Pinto, RD (Author) Other; Nascimento, DS (Author) Other; do Vale, A (Author) Other; dos Santos, NMS (Author) Other

Journal

Title: Veterinary Immunology and Immunopathology

Vol. 110

Pages: 169-177

ISSN: 0165-2427

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 15 Citations

Scopus - 17 Citations

Other information

Authenticus ID: P-004-MRA

DOI: 10.1016/j.vetimm.2005.11.005

Abstract (EN): In this work, the gene and cDNA of the sea bass CD8 alpha have been isolated and characterized. The coding sequence has an ORF of 666 bp. It retains the Ig motif that interacts with MHC and the two cysteines responsible for an intra-chain disulfide bridge. The hinge region contains the two essential cysteines involved in dimerization. The transmembrane region is well conserved in all analysed sequences. Similar to other teleosts, the cytoplasmic region lacks the consensus p56(1ck) motif common in higher vertebrates. Analysis of the expression pattern using RT-PCR shows the highest expression in the thymus. Like in the human gene, the sea bass CD8 alpha genomic structure is organized into six exons, which roughly correspond to separate functional domains of the protein. Southern blotting shows that CD8 alpha exists as a single copy gene. Together, these results support the concept that the basic structure of CD8a has been maintained through evolution.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9