Consistent Long-Term Therapeutic Efficacy of Human Umbilical Cord Matrix-Derived Mesenchymal Stromal Cells After Myocardial Infarction Despite Individual Differences and Transient Engraftment

• Type: Article in International Scientific Journal
• Date: 2021

Authors

Laundos, TL (Author) Other; Vasques Novoa, F (Author) FMUP; Gomes, RN (Author) Other; Sampaio Pinto, V (Author) Other; Cruz, P (Author) Other; Cruz, H (Author) Other; Santos, JM (Author) Other; Barcia, RN (Author) Other; Pinto do O, P (Author) Other; Nascimento, DS (Author) ICBAS

Journal

Title: Frontiers in Cell and Developmental Biology

Vol. 9

Publisher: Frontiers Media

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 1 Citation

Other information

Authenticus ID: P-00T-H2R

DOI: 10.3389/fcell.2021.624601

Abstract (EN): Human mesenchymal stem cells gather special interest as a universal and feasible add-on therapy for myocardial infarction (MI). In particular, human umbilical cord matrix-derived mesenchymal stromal cells (UCM-MSC) are advantageous since can be easily obtained and display high expansion potential. Using isolation protocols compliant with cell therapy, we previously showed UCM-MSC preserved cardiac function and attenuated remodeling 2 weeks after MI. In this study, UCM-MSC from two umbilical cords, UC-A and UC-B, were transplanted in a murine MI model to investigate consistency and durability of the therapeutic benefits. Both cellular products improved cardiac function and limited adverse cardiac remodeling 12 weeks post-ischemic injury, supporting sustained and long-term beneficial therapeutic effect. Donor associated variability was found in the modulation of cardiac remodeling and activation of the Akt-mTOR-GSK3 beta survival pathway. In vitro, the two cell products displayed similar ability to induce the formation of vessel-like structures and comparable transcriptome in normoxia and hypoxia, apart from UCM-MSCs proliferation and expression differences in a small subset of genes associated with MHC Class I. These findings support that UCM-MSC are strong candidates to assist the treatment of MI whilst calling for the discussion on methodologies to characterize and select best performing UCM-MSC before clinical application.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 15