Electrochemical and optical biosensing platforms for the immunorecognition of hazelnut Cor a 14 allergen

• Type: Article in International Scientific Journal
• Date: 2021

Authors

Costa, R (Author) FCUP; Joana Costa (Author) FFUP; Sagastizábal, I (Author) Other; Brandao, ATSC (Author) FCUP; Moreira, P (Author) Other; Mafra, I (Author) Other; Silva, F (Author) FCUP; Carlos M Pereira (Author) FCUP

Journal

Title: Food Chemistry

Vol. 361

ISSN: 0308-8146

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 19 Citations

Scopus - 20 Citations

Other information

Authenticus ID: P-00V-1MZ

DOI: 10.1016/j.foodchem.2021.130122

Abstract (EN): Two immunosensors were advanced to target hazelnut Cor a 14 based on electrochemical and optical transduction. Both approaches were developed with two types of custom-made antibodies, namely anti-Cor a 14 IgG (rabbit) and anti-Cor a 14 IgY (hen's egg) targeting the Cor a 14 allergen. Antibody immobilisation was performed via EDC/NHS onto disposable screen-printed electrodes. The detection limit (LOD) of the electrochemical immunoassay for Cor a 14 was 5-times lower than the optical, being down to 0.05 fg mL-1 with a dynamic range of 0.1 fg mL-1 to 0.01 ng mL-1. Antibody selectivity was verified against non-target 2S albumins (potential crossreactive plant species). Anti-Cor a 14 IgY exhibited the best specificity, presenting minor cross-reactivity with peanut/walnut. Preliminary results of the application of anti-Cor a 14 IgY electrochemical immunosensor to incurred foods established a LOD of 1 mg kg- 1 of hazelnut in wheat (0.16 mg kg- 1 hazelnut protein).

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11