L-Asparaginase-Based Biosensors

• Type: Article in International Scientific Journal
• Date: 2021

Authors

João C. F. Nunes (Author) Other; Raquel O. Cristóvão (Author) FEUP; Valéria C. Santos-Ebinuma (Author) Other; Joaquim Luís Faria (Author) FEUP; Cláudia G. Silva (Author) FEUP; Márcia C. Neves (Author) Other; Mara G. Freire (Author) Other; Ana P. M. Tavares (Author) Other

Journal

The Journal is awaiting validation by the Administrative Services.

Title: Encyclopedia

Vol. 1

Pages: 848-858

ISSN: 2673-8392

Indexing

ISI Web of Science

Scopus

Associated Projects

NanoPurAsp - Desenvolvimento de nanomateriais sustentáveis para a purificação de fármacos antileucémicos

Other information

Authenticus ID: P-00V-KNM

DOI: 10.3390/encyclopedia1030065

Abstract (EN): <jats:p>L-asparaginase (ASNase) is an aminohydrolase enzyme widely used in the pharmaceutical and food industries. Although currently its main applications are focused on the treatment of lymphoproliferative disorders such as acute lymphoblastic leukemia (ALL) and acrylamide reduction in starch-rich foods cooked at temperatures above 100 °C, its use as a biosensor in the detection and monitoring of L-asparagine levels is of high relevance. ASNase-based biosensors are a promising and innovative technology, mostly based on colorimetric detection since the mechanism of action of ASNase is the catalysis of the L-asparagine hydrolysis, which releases L-aspartic acid and ammonium ions, promoting a medium pH value change followed by color variation. ASNase biosensing systems prove their potential for L-asparagine monitoring in ALL patients, along with L-asparagine concentration analysis in foods, due to their simplicity and fast response.</jats:p>

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11