Desmoplastic small round cell tumour: Cytological and immunocytochemical features

• Type: Article in International Scientific Journal
• Date: 2005

Authors

Granja, NM (Author) Other; Begnami, MD (Author) Other; Bortolan, J (Author) Other; Longatto Filho, A (Author) Other; Fernando Schmitt (Author) FMUP

Journal

Title: CytoJournal

Vol. 2

Final page: 6

ISSN: 0974-5963

Publisher: Scientific Scholar

Indexing

Scopus - 42 Citations

Other information

Authenticus ID: P-007-D0S

DOI: 10.1186/1742-6413-2-6

Abstract (EN): Background: Desmoplastic small round cell tumor (DSRCT) is a rare and highly aggressive neoplasm. The cytological diagnosis of these tumors can be difficult because they show morphological features quite similar to other small round blue cells tumors. We described four cases of DSRCT with cytological sampling: one obtained by fine needle aspiration biopsy (FNAB) and three from serous effusions. The corresponding immunocytochemical panel was also reviewed. Methods: Papanicolaou stained samples from FNAB and effusions were morphologically described. Immunoreaction with WT1 antibody was performed in all cytological samples. An immunohistochemical panel including the following antibodies was performed in the corresponding biopsies: 34BE12, AE1/AE3, Chromogranin A, CK20, CK7, CK8, Desmin, EMA, NSE, Vimentin and WT1. Results: The smears showed high cellularity with minor size alteration. Nuclei were round to oval, some of them with inconspicuous nucleoli. Tumor cells are clustered, showing rosette-like feature. Tumor cells in effusions and FNA were positive to WT1 in 3 of 4 cytology specimens (2 out 3 effusions and one FNA). Immunohistochemical reactions for vimentin, NSE, AE1/AE3 and WT1 were positive in all cases in tissue sections. Conclusion: The use of an adjunct immunocytochemical panel coupled with the cytomorphological characteristics allows the diagnosis of DSRCT in cytological specimens. © 2005 Granja et al; licensee BioMed Central Ltd.

Language: English

Type (Professor's evaluation): Scientific