Microsatellite instability in medullary breast carcinomas

• Type: Article in International Scientific Journal
• Date: 1999

Authors

Fernando Schmitt (Author) Other; soares, r (Author) FMDUP; Gobbi, H (Author) Other; Milanezzi, F (Author) Other; Santos-Silva F (Author) Other; Cirnes, L (Author) Other; Costa, C (Author) Other; Seruca, R (Author) Other

Journal

Title: International Journal of Cancer

Vol. 82

Pages: 644-647

ISSN: 0020-7136

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 24 Citations

Scopus - 27 Citations

Other information

Authenticus ID: P-001-3M4

DOI: 10.1002/(sici)1097-0215(19990827)82:5<644::aid-ijc5>3.0.co;2-s

Abstract (EN): Microsatellite instability (MSI) has been reported to occur in a wide variety of sporadic tumours, such as colorectal and gastric cancers. MSI positivity has been associated with a particular clinico-pathologic profile, including the presence of abundant lymphoid infiltration, poor differentiation and a relatively good outcome for the patients. Since medullary breast carcinomas (MBCs) share these clinico-pathologic features with the MSI-positive tumours described above, we evaluated MSI in this particular histologic type of breast cancer. DNA of 24 MBC cases was extracted from formalin-fixed, paraffin-embedded tissue. The presence of MSI was analysed using BAT-26. We also searched mutations in 2 target genes: TGF-beta RII and BAX. Five cases of the series were also analysed for I (CA) dinucleotide tandem repeat sequence (D1S158), 8 tetranucleotide repeat sequences (D3S1358, D5S818, D7S820, D8S1179, D13S317, D21S11, FGA and VWA) and I pentanucleotide repeat (dAAAAT), localized in intron I of p53 gene. We found 2 carcinomas (8.3%) with BAT-26 instability. None of the cases had mutations in the "target genes", TGF-beta RII and BAX, including the 2 cases with BAT-26 instability. No MSI was observed using the panel of tetra- and pentanucleotide markers. Loss of heterozygosity was found in some loci. No significant difference in mean MIB-I index according to RER status was observed. The low frequency of MSI in MBC is similar to that of other histologic types of breast cancer, Although MBCs share some clinico-pathologic features with colorectal and gastric carcinomas, which exhibit a high frequency of MSI, the underlying genetic events leading to this breast tumour are different from those leading to tumours of the digestive tract. int, J. Cancer 82:644-647, 1999. (C) 1999 Wiley-Liss, Inc.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4