Characterization of oligoadenylate synthetase-1 expression in rat mammary gland and prostate: effects of 17 beta-estradiol on the regulation of OAS1g in both tissues

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Maia, CJB (Author) Other; Socorro, S (Author) Other; Fernando Schmitt (Author) FMUP; Santos, CRA (Author) Other

Journal

Title: Molecular and Cellular Biochemistry

Vol. 314

Pages: 113-121

ISSN: 0300-8177

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 5 Citations

Other information

Authenticus ID: P-003-Y98

DOI: 10.1007/s11010-008-9771-z

Abstract (EN): OAS1 belongs to a protein family of interferon-induced enzymes characterized by their ability to catalyze the synthesis of 2'-5'-linked oligomers of adenosine from ATP (2-5A). 2-5A bind to the latent Ribonuclease L (RNase L), which subsequently dimerizes into the active form, acquiring the capacity of cleaving cellular and viral mRNA. Several studies indicate that OAS1 is an important inducer of apoptosis in human cancer cells and that it may be regulated by 17 beta-estradiol (E(2)). The aim of this study was to characterize OAS1 gene expression in rat mammary gland and prostate, and to analyze its regulation by E(2) in both tissues. It is demonstrated that OAS1g is the most abundant OAS1 gene expressed in both tissues, and that OAS1 protein is present in the nucleus of rat mammary gland and prostate epithelial cells. In addition, it is shown by Real Time PCR that OAS1g is up-regulated by E(2) in rat mammary gland, but down-regulated in prostate, suggesting that the OAS1g gene may be related to estrogen dependent pathways in rat mammary gland and prostate physiology.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9