Tissue microarrays for testing basal biomarkers in familial breast cancer cases

• Type: Article in International Scientific Journal
• Date: 2007

Authors

Dufloth, RM (Author) Other; Matos, I (Author) Other; Fernando Schmitt (Author) FMUP; Zeferino, LC (Author) Other

Journal

Title: Sao Paulo Medical Journal

Vol. 125

Pages: 226-230

ISSN: 1516-3180

Publisher: Associação Paulista de Medicina

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 3 Citations

Other information

Authenticus ID: P-004-8WD

Abstract (EN): CONTEXT AND OBJECTIVE: The proteins p63, p-cadherin and CK5 are consistently expressed by the basal and myoepithelial cells of the breast, although their expression in sporadic and familial breast cancer cases has yet to be fully defined. The aim here was to study the basal immunoprofile of a breast cancer case series using tissue microarrary technology. DESIGN AND SETTING: This was a cross-sectional study at Universidade Estodual de Compinas, Brazil, and the Institute of Pathology and Molecular Immunology, Porto, Portugal. METHODS: Immunohistochemistry using the antibodies l CK5 and p-cadherin, and also estrogen receptor (ER) and Human Epidermal Receptor Growth Factor 2 (HER2), was performed on 168 samples from a breast cancer case series. The criteria for identifying women at high risk were based on those of the Breast Cancer Linkage Consortium. RESULTS: Familial tumors were more frequently positive for the p-cadherin (p = 0.0004), p63 (p < 0.0001) and CK5 (p < 0.0001) than was sporadic cancer. Moreover, familial tumors had coexpression of the basal biomarkers CK5+/p63+, grouped two by two (OR = 34.34), while absence of coexpression (OR = 0.13) was associated with the sporadic cancer phenotype. CONCLUSION: Familial breast cancer was found to be associated with basal biomarkers, using tissue microarray technology. Therefore, characterization of the familial breast cancer phenotype will improve the understanding of breast carcinogenesis.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5