Potential use of loss of heterozygosity in pleural effusions of breast cancer metastases using the microsatellite marker of the 16q22.1 region of the CDH1 gene

• Type: Article in International Scientific Journal
• Date: 2005

Authors

Granja, ND (Author) Other; Ricardo, SAV (Author) Other; Longatto, A (Author) Other; Ferreira, VA (Author) Other; Bedrossian, CWM (Author) Other; Wiley, EL (Author) Other; Fernando Schmitt (Author) FMUP

Journal

Title: Analytical and Quantitative Cytology and Histology

Vol. 27

Pages: 61-66

ISSN: 0884-6812

Publisher: Science Printers and Publishers

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 5 Citations

Other information

Authenticus ID: P-000-3WX

Abstract (EN): OBJECTIVE: To evaluate the presence of allelic loss in 16q22.1, including the locus of E-cadherin, in pleural effusions in breast cancer patients. STUDY DESIGN: Molecular analysis of DNA was performed using a DNA extraction kit (NucleoSpin, Macherey-Nagel, Germany). Loss of heterozygosity (LOH) in primary tumors and pleural effusions was analyzed using a microsatellite marker of the CDH1 gene, D16S265, described in previous studies.(15) LOH was evaluated by radioactive polymerase chain reaction assay in 17 samples of pleural effusions and breast tissues (primary tumors and nonneoplastic adjacent tissue) from breast cancer patients: 7 positive for neoplastic cells, 6 suspected and 4 cases without evidence of neoplastic cells in the effusions. RESULTS: Thirteen cases (76%) were informative. LOH was detected in 5 cases (38.5%). In 3 of them LOH was detected only in the cytologic sample, and in 2 of them LOH was detected in the primary tumor and cytologic sample. CONCLUSION: Results shod, that LOH in the CDH1 gene cart identify tumor cells in pleural effusions When morphologic analysis is difficult.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6