Detection of N-myc amplification in neuroblastomas using Southern blotting on fine needle aspirates

• Type: Article in International Scientific Journal
• Date: 2001

Authors

Barroca, H (Author) Other; Carvalho, JL (Author) Other; da Costa, MJG (Author) Other; Cirnes, L (Author) Other; Seruca, R (Author) FMUP; Fernando Schmitt (Author) FMUP

Journal

Title: Acta Cytologica

Vol. 45

Pages: 169-172

ISSN: 0001-5547

Publisher: Karger

Indexing

ISI Web of Knowledge - 14 Citations

Scopus - 21 Citations

Other information

Authenticus ID: P-000-W5G

Abstract (EN): OBJECTIVE: To verify the possibility of detecting N-myc amplification in fine needle aspiration (FNA) cytology from neuroblastomas by the Southern blotting technique. STUDY DESIGN: Fifteen neuroblastomas diagnosed by FNA in the Department of Pathology, Hospital de S. Joao, between 1990 and 1998, were studied for N-myc amplification using the Southern blotting technique in cytologic and histologic material. RESULTS: DNA extraction from the cytologic material was performed in all cases (N = 15). In two cases the quality/quantity of the DNA did not allow the study of N-myc status by the Southern blotting technique. We detected N-myc amplification in 1 of 13 patients (7.6%) from whom material was available for genetic study. CONCLUSION: It is possible to use the Southern blotting technique to demonstrate N-myc amplification in material obtained from FNA of neuroblastomas.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4