Characterization and spatial relationships of the hepatic vascular-biliary tracts, and their associated pancreocytes and macrophages, in the model fish guppy (Poecilia reticulata): A study of serial sections by light microscopy

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Sousa, S (Author) Other; rocha, mj (Author) ICBAS; rocha, e (Author) ICBAS

Journal

Title: TISSUE & CELL

Vol. 50

Pages: 104-113

ISSN: 0040-8166

Publisher: CHURCHILL LIVINGSTONE

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 4 Citations

Other information

Authenticus ID: P-00N-SDN

DOI: 10.1016/j.tice.2017.12.009

Abstract (EN): The guppy is a tropical fish that has been used as an experimental model organism in science. It is a species well adapted to the natural environment and that can support adverse environmental conditions, and so, at occasions, its presence can be indicative of environmental disturbances. Moreover, as the liver is very important when studying fish diseases, the knowledge of normal microanatomy is essential to assess histological changes, e.g., related to environmental change or toxic pollutants. The target organ of this histological study is the liver. The main objective is to contribute to the identification of anatomical and structural variations of this organ in different teleost species. We studied the distribution and spatial organization of the different types of blood vessels and biliary ducts and the relationships between them are established. For this, each liver was totally sectioned and the serial sections inspected in detail. The guppy liver presented intra-hepatic pancreatic tissue and so reported its association with the vascular and biliary elements. We observed that the input of afferent vessels (i.e., bringing blood into the liver) occur not only in the hilum but pierce and enter the organ at various points. Within the liver, venous vessels and bile ducts are seen, isolated or associated as venous-arteriolar tracts (VAT), and venous-biliary-arteriolar tracts (VBAT). Sometimes, pancreocytes appear within the liver surrounding isolated veins, forming venous tract with pancreatic acini (VT-P), or dual associations with afferent vessels, forming venous-arteriolar tracts with pancreatic acini (VAT-P). Intrahepatic pancreatic ducts were tiny and rare, putting in question the functional role of the acini. Contrary to other fish species, we did not spot isolated arterioles and associations between these and biliary ducts (BAT). We found aggregates of macrophages, namely associated with afferent and efferent (i.e., draining blood out) venous vessels; the latter fact not commonly reported in other fish species. There was a reduced arterialization of the organ (as arterioles were extremely rare), contrasting with an over predominance of a random distribution of the venous vascularization. The guppy differs to some extent from other previously studied models, highlighting the importance of making this kind of study to offer specific frameworks that can explain specific physiological processes or avoid misinterpretations; for instance about gene expression, as the whole liver specific expression will reflect the presence of hepatocytes and pancreocytes as well.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10