EVIDENCE THAT CHLOROETHYLCLONIDINE CAN CONTRACT THE DOG SAPHENOUS-VEIN VIA A PROTEIN KINASE-C-DEPENDENT MECHANISM

• Type: Article in International Scientific Journal
• Date: 1994

Authors

José Pedro Nunes (Author) FMUP; Alberto Mota (Author) FMUP

Journal

Title: Archives Internationales de Pharmacodynamie et de Therapie

Vol. 327

Pages: 142-151

ISSN: 0003-9780

Publisher: Blackwell

Indexing

ISI Web of Knowledge - 1 Citation

Scopus - 2 Citations

Other information

Authenticus ID: P-001-KAA

Abstract (EN): Chloroethylclonidine has recently been shown to produce concentration-dependent contractions in the dog saphenous vein. These contractions are very sensitive to cooling, yohimbine and rauwolscine, and moderately sensitive to prazosin. The effects of compound H-7 (a protein kinase inhibitor) and isradipine (a calcium channel blocker) on the contractions to chloroethylclonidine, UK14304 and phenylephrine were studied in the dog saphenous vein. Compound H-7 (20 mumol/l) produced a very marked (85 %) inhibition of chloroethylclonidine-induced contractions, a moderate (48 %) inhibition of UK14304-induced contractions and a slight (19 %) inhibition of phenylephrine-induced contractions. Isradipine (1 mumol/l) produced a moderate (49 %) inhibition of chloroethylclonidine-induced contractions and a slight (15 %) inhibition of phenylephrine-induced contractions. Phorbol 12,13-dibutyrate (1 mumol/l) produced a contraction that reached 61.1 +/- 3.2 % of the maximum and was inhibited moderately (46 %) by compound H-7. It is suggested that, in the dog saphenous vein, the receptor activated by chloroethylclonidine may be coupled to protein kinase C.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10