In silico and experimental evaluation of DNA-based detection methods for the ability to discriminate almond from other Prunus spp.

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Brezna, B (Author) Other; Smid, J (Author) Other; Joana Costa (Author) FFUP; Radvanszky, J (Author) Other; Mafra, I (Author) Other; Kuchta, T (Author) Other

Journal

Title: Molecular and Cellular Probes

Vol. 29

Pages: 99-115

ISSN: 0890-8508

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 1 Citation

Scopus - 2 Citations

Other information

Authenticus ID: P-00A-8R9

DOI: 10.1016/j.mcp.2014.11.006

Abstract (EN): Ten published DNA-based analytical methods aiming at detecting material of almond (Prunus dulcis) were in silica evaluated for potential cross-reactivity with other stone fruits (Prunus spp.), including peach, apricot, plum, cherry, sour cherry and Sargent cherry. For most assays, the analysis of nucleotide databases suggested none or insufficient discrimination of at least some stone fruits. On the other hand, the assay targeting non-specific lipid transfer protein (Roder et al., 2011, Anal Chim Acta 685:74-83) was sufficiently discriminative, judging from nucleotide alignments. Empirical evaluation was performed for three of the published methods, one modification of a commercial kit (SureFood allergen almond) and one attempted novel method targeting thaumatin-like protein gene. Samples of leaves and kernels were used in the experiments. The empirical results were favourable for the method from Roder et al. (2011) and a modification of SureFood allergen almond kit, both showing cross-reactivity <10(-3) compared to the model almond.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 17