Content of hypericins from plants and invitro shoots of Hypericum undulatum Schousb. ex Willd

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Nuno Rainha (Author) Other; Elisabete Lima (Author) Other; Jose Baptista (Author) Other; Manuel Fernandes Ferreira (Author) FCUP

Journal

Title: Natural Product Research

Vol. 27

Pages: 869-879

ISSN: 1478-6419

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 0 Citations

ISI Web of Science

Scopus - 2 Citations

Pubmed / Medline

Scientific classification

FOS: Natural sciences > Chemical sciences

CORDIS: Natural sciences

Other information

Authenticus ID: P-005-0TZ

DOI: 10.1080/14786419.2012.688051

Resumo (PT): This study reports the first quantification study of pseudohypericin (PsHyp) and hypericin (Hyp) in Hypericum undulatum Schousb. ex Willd in vitro cultures developed by a Portuguese company. Both compounds were quantified by highperformance liquid chromatography and their levels were compared with those in commercial samples of Hypericum perforatum. PsHyp was found to be the major naphthodianthrone of H. undulatum, with an average ratio of 3.73:1 compared to Hyp. Significant statistical differences were found between the content of Hyp and PsHyp in H. undulatum regenerated shoots compared to in vivo samples. The mean concentration of total Hyps varied from 178.41 to 358.93 mgg1 dry extract in H. undulatum regenerated shoots, which is on average two to three times less than naphthodianthrone levels found in H. undulatum in vivo and H. perforatum commercial samples. However, none of the analysed samples presented the levels of Hyps required by the European and United States Pharmacopoeias.

Abstract (EN): This study reports the first quantification study of pseudohypericin (PsHyp) and hypericin (Hyp) in Hypericum undulatum Schousb. ex Willd invitro cultures developed by a Portuguese company. Both compounds were quantified by high-performance liquid chromatography and their levels were compared with those in commercial samples of Hypericum perforatum. PsHyp was found to be the major naphthodianthrone of H. undulatum, with an average ratio of 3.73:1 compared to Hyp. Significant statistical differences were found between the content of Hyp and PsHyp in H. undulatum regenerated shoots compared to invivo samples. The mean concentration of total Hyps varied from 178.41 to 358.93 mu gg(-1) dry extract in H. undulatum regenerated shoots, which is on average two to three times less than naphthodianthrone levels found in H. undulatum invivo and H. perforatum commercial samples. However, none of the analysed samples presented the levels of Hyps required by the European and United States Pharmacopoeias.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11