Association and Phase Behavior of Cholic Acid-Modified Dextran and Phosphatidylcholine Liposomes

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Guangyue Bai (Author) Other; Marieta Nichifor (Author) Other; Margarida Bastos (Author) FCUP

Journal

Title: Journal Of Physical Chemistry Letters

Vol. 1 No. 6

Pages: 932-936

ISSN: 1948-7185

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 16 Citations

Scopus - 15 Citations

Scientific classification

CORDIS: Physical sciences > Chemistry > Applied chemistry ; Physical sciences > Chemistry > Physical chemistry

FOS: Engineering and technology > Materials engineering

Other information

Authenticus ID: P-003-88M

DOI: 10.1021/jz100087k

Abstract (EN): The interaction between liposomes (1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC)) and a hydrophobically modified water-soluble polymer (HMP; a bile acid-modified dextran)has been investigated by isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC), combined with turbidity measurement and cryogenic scanning electron microscopy (cryo-SEM). The thermodynamic information on the association (enthalpy of interaction, enthalpy of transition of mixed vesicles to mixed micelle-like aggregates) was obtained from lit. Further, the phase behavior for the system could be derived from the lit measurements, and be confirmed by turbidity and cryo-SEM. The effect of cholic acid (CA) side groups on the ordered arrangement of DMPC bilayers Was studied by DSC, by following the changes they induce in the gel-to-liquid crystalline liposome phase transition. The DSC results were in excellent agreement. with the interpretation proposed for the ITC results. The morphology of the aggregates; as characterized by cryo-SEM, is in line with the proposed aggregate morphologies.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5