Regular voluntary running inhibits androgen-independent prostate cancer growth in mice

• Type: Article in International Scientific Journal
• Date: 2021

Authors

Mário Esteves (Author) Other; Carina Silva (Author) Other; Sofia S. Pereira (Author) Other; Tiago Morais (Author) Other; Ângela Moreira (Author) Other; Madalena M. Costa (Author) Other; Mariana P. Monteiro (Author) Other; José A. Duarte (Author) FADEUP

Journal

Title: Journal of Physical Activity & Health

Vol. 18 No. 6

Pages: 653-659

ISSN: 1543-3080

Publisher: Human Kinetics Publishers, Inc.

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 0 Citations

Other information

Authenticus ID: P-00V-0MY

DOI: 10.1123/jpah.2020-0761

Abstract (EN): Introduction: Benefits of regular physical exercise were demonstrated as preventive and coadjuvant nonpharmacological anticancer therapy. However, the role of exercise in modulating prostate cancer behavior has yet to be established. Methods: Prostate tumors were induced in C57BL/6 male mice (n = 28) by subcutaneous inoculation of a suspension of murine androgen-independent RM1 cells (1.5 x 10(5) cells/500 mu L phosphate-buffered saline) in the dorsal region. Mice were randomly allocated into 2 study groups: sedentary tumor-induced (n = 14) and exercised tumor-induced (n = 14). Exercise consisted of voluntary running in wheeled cages. Mice (n = 7 per group) were sacrificed either 14 or 28 days after cell inoculation to evaluate tumor weight and percentage of area occupied by immunohistochemistry stained cells for Ki-67 and TdT-mediated dUTP-biotin nick end labeling, used as surrogate markers of cell proliferation and apoptosis, respectively. Results: Compared with sedentary tumor-induced mice, the tumors developed by exercised tumor-induced mice were significantly smaller at 14 days (0.17 [0.12] g vs 0.48 [0.24] g, P < .05) and at 28 days (0.92 [0.73] g vs 2.09 [1.31] g, P < .05), with smaller Ki-67 and greater TdT-mediated dUTP-biotin nick end-labeling stained areas (P < .05). Conclusion: These results suggest that regular voluntary running inhibits prostate cancer cell growth by reducing cell proliferation and enhancing apoptosis.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7