Flavonoid transport across RBE4 cells: A blood-brain barrier model

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Faria, Ana (Author) FCNAUP; Pestana, D (Author) Other; Teixeira, D (Author) Other; Azevedo, J (Author) Other; Victor de Freitas (Author) FCUP; Nuno Mateus (Author) FCUP; Conceicao Calhau (Author) FMUP

Journal

Title: CELLULAR & MOLECULAR BIOLOGY LETTERS

Vol. 15 No. 2

Pages: 234-241

ISSN: 1425-8153

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 109 Citations

Scopus - 123 Citations

Pubmed / Medline

Scientific classification

FOS: Engineering and technology > Chemical engineering

CORDIS: Natural sciences > Biological sciences > Biology > Molecular biology ; Physical sciences > Chemistry > Biochemistry > Cytochemistry ; Physical sciences > Chemistry > Analytical chemistry > Spectrochemistry

Other information

Authenticus ID: P-003-5WT

DOI: 10.2478/s11658-010-0006-4

Abstract (EN): There is a growing interest in dietary therapeutic strategies to combat oxidative stress-induced damage to the Central Nervous System (CNS), which is associated with a number of pathophysiological processes, including Alzheimer's and Parkinson's diseases and cerebrovascular diseases. Identifying the mechanisms associated with phenolic neuroprotection has been delayed by the lack of information concerning the ability of these compounds to enter the CNS. The aim of this study was to evaluate the transmembrane transport of flavonoids across RBE-4 cells (an immortalized cell line of rat cerebral capillary endothelial cells) and the effect of ethanol on this transport. The detection and quantification of all of the phenolic compounds in the studied samples (basolateral media) was performed using a HPLC-DAD (Diode Array Detector). All of the tested flavonoids (catechin, quercetin and cyanidin-3-glucoside) passed across the RBE-4 cells in a time-dependent manner. This transport was not influenced by the presence of 0.1% ethanol. In conclusion, the tested flavonoids were capable of crossing this blood-brain barrier model.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8