New improved method for fructooligosaccharides production by Aureobasidium pullulans

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Domingues, A. (Author) Other; Nobre, C. (Author) Other; Rodrigues, L.R. (Author) Other; Peres, A.M. (Author) Other; Torres, Duarte (Author) FCNAUP; Rocha, I. (Author) Other; Lima, N. (Author) Other; Teixeira, José (Author) FCNAUP

Journal

Title: Carbohydrate Polymers

Vol. 89 No. 4

Pages: 1174-1179

ISSN: 0144-8617

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 52 Citations

ISI Web of Science

Scopus - 64 Citations

Scientific classification

FOS: Agrarian Sciences > Agricultural biotechnology

CORDIS: Technological sciences > Technology > Biotecnology > Food biotechnology

Other information

Authenticus ID: P-002-7S5

DOI: 10.1016/j.carbpol.2012.03.091

Abstract (EN): Fructooligosaccharides are prebiotics with numerous health benefits within which the improvement of gut microbiota balance can be highlighted, playing a key role in individual health. In this study, an integrated one-stage method for FOS production via sucrose fermentation by Aureobasidium pullulans was developed and optimized using experimental design tools. Optimization of temperature and agitation speed for maximizing the FOS production was performed using response surface methodology. Temperature was found to be the most significant parameter. The optimum fermentation conditions were found to be 32 degrees C and 385 rpm. Under these conditions, the model predicted a total FOS production yield of 64.7 g(FOS)/g(sucrose). The model was validated at optimal conditions in order to check its adequacy and accuracy and an experimental yield of 64.1 (+/- 0.0)g(FOS)/g(sucrose) was obtained. A significant improvement of the total FOS production yields by A. pullulans using a one-stage process was obtained. (C) 2012 Elsevier Ltd. All rights reserved.

Language: Portuguese

Type (Professor's evaluation): Scientific

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