Liver transcriptome resources of four commercially exploited teleost species

• Type: Other Publications
• Date: 2020

Authors

Machado, AM (Author) Other; Munoz Merida, A (Author) Other; Fonseca, E (Author) Other; Verissimo, A (Author) FCUP; Pinto, R (Author) Other; Felicio, M (Author) Other; da Fonseca, RR (Author) Other; Froufe, E (Author) Other; Filipe F C Castro (Author) FCUP

Other information

Authenticus ID: P-00S-CP3

DOI: 10.1038/s41597-020-0565-9

Abstract (EN): The generation ofomicresources is central to develop adequate management strategies for species with economic value. Here, we provide high-coverage RNA-seq datasets of liver tissue (containing between 80,2 and 88,4 million of paired-end reads) from four wildtype teleost species with high commercial value:Trachurus trachurus(TTR; Atlantic horse mackerel),Scomber scombrus(SSC; Atlantic mackerel),Trisopterus luscus(TLU; pout), andMicromesistius poutassou(MPO; blue whiting). A comprehensive assembly pipeline, usingde novosingle and multi-kmer assembly approaches, produced 64 single high-quality liver transcriptomes - 16 per species. The final assemblies, with N50 values ranging from 2,543-3,700 bp and BUSCO (Benchmarking Universal Single-Copy Orthologs) completeness values between 81.8-86.5% of the Actinopterygii gene set, were subjected to open reading frame (ORF) prediction and functional annotation. Our study provides the first transcriptomic resources for these species and offers valuable tools to evaluate both neutral and selected genetic variation among populations, and to identify candidate genes for environmental adaptation assisting in the investigation of the effects of global changes in fisheries.

Language: English

Type (Professor's evaluation): Scientific