Grape pectic polysaccharides stabilization of anthocyanins red colour: Mechanistic insights

• Type: Article in International Scientific Journal
• Date: 2021

Authors

Fernandes, A (Author) Other; Raposo, F (Author) Other; Evtuguin, DV (Author) Other; Fonseca, F (Author) Other; Ferreira da Silva, F (Author) Other; Nuno Mateus (Author) FCUP; Coimbra, MA (Author) Other; Victor de Freitas (Author) FCUP

Journal

Title: Carbohydrate Polymers

Vol. 255

ISSN: 0144-8617

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 26 Citations

Scopus - 28 Citations

Other information

Authenticus ID: P-00T-7GT

DOI: 10.1016/j.carbpol.2020.117432

Abstract (EN): Grape pectic polysaccharides-malvidin 3-O-beta-D-glucoside binding was studied, aiming to unveil the impact of structural diversity of polysaccharides on anthocyanins-polysaccharides interactions. Polysaccharides were extracted with solutions of imidazole (ISP) and carbonate at 4 degrees C (CSP-4 degrees C) and room temperature (CSP-RT) and also recovered from the dialysis supernatant of the remaining cellulosic residue after the aqueous NAOH extraction of hemicellulosic polysaccharides (Sn-CR). Polysaccharides richer in homogalacturonan domains, like those present in the CSP-4 degrees C fraction had approximately 50-fold higher binding affinity to malvidin-3-O- beta-D-glucoside, than polysaccharides with side chains (as ISP and CSP-RT extractable polysaccharides). CSP-4 degrees C polysaccharides showed a positive effect on malvidin-3-O beta-D-glucoside colour fading. Hydration equilibrium constant of malvidin-3-O- beta-D-glucoside in the presence of CSP-4 degrees C polysaccharides was higher, showing the preferential stabilization of the flavylium cation. The results showed that anthocyanins colour stabilization can be promoted by pectic polysaccharide structures such as those extracted by cold carbonate.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10