Interaction of a Procyanidin Mixture with Human Saliva and the Variations of Salivary Protein Profiles over a 1-Year Period

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Guerreiro, C (Author) Other; Jesus, M (Author) Other; Brandao, E (Author) Other; Nuno Mateus (Author) FCUP; Victor de Freitas (Author) FCUP; Susana Soares (Author) FCUP

Journal

Title: Journal of Agricultural and Food Chemistry

Vol. 68

ISSN: 0021-8561

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 7 Citations

Other information

Authenticus ID: P-00T-2WS

DOI: 10.1021/acs.jafc.0c05722

Abstract (EN): Procyanidins are widely associated with astringency perception and promptly interact/precipitate salivary proteins (SPs). In this work, the SP profile of 17 volunteers was monitored for 1 year, focusing on the SP families most related to astringency [aPRPs (acidic proline-rich proteins), bPRPs (basic proline-rich proteins), gPRPs (glycosylated proline-rich proteins), cystatins, P-B peptide, and statherin]. Although the total SP content remained constant, bPRPs showed high variability. Saliva from 5 volunteers was selected, each individual's saliva presenting a prominence in one of the referred SP families; each was used to interact with grape seed procyanidin oligomeric fraction. Independent of the prominences, a total depletion in statherin and P-B peptide was observed. These subjects performed a sensory assay and the limit of detection for astringency was determined. Overall, the specificity of SP toward procyanidins seemed to be more important in the interactions than the total SP content. The highest reactivity toward SPs was observed for epicatechin gallate, procyanidin dimers B7, B2g, and trimer C1.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9